![]() ![]() At that point I finish my consensus sequence. Select both files with the mouse by dragging it over the file names at the left. Delete and copy the data of highlighted sequence. Each line in the trace is colour-coded to match the colour that one of the 4 bases is displayed in. ![]() Save the file as text only and make sure it has the correct file extension. Select them all control-acopy to clipboard control-cgo back to BioEdit, to paste these names over the existing ones. Save the edited file to the desktop, or preferably, your own disk or network account. Select to the end including the current residue. There are 4 disks containing sequence files. Note that sequences after bases become increasingly unreliable, and are not worth spending much time on. This highlights any columns that have different bases. Now your BioEdit file has all the forwards and reverses, with the. The reason why I paste them to a new file first is that importing from the clipboard File, Import from Clipboard will place them at the bottom of your file, which is usually not where I want them be. Since this may interfere with analysis of the sequence, these will have to be edited out. These should show an almost exact match to the forward titorial reverse sequence. These are my preferences, you can use these or change them whatever you prefer. Guide to editing sequences with Chromas and BioEdit See sequence analysis references for full map. MEGA also has an alignment editor, but I’ve not really used it very much. BioEdit can also edit chromatograms, but I find Chromas to be nicer. BioEdit is a mouse-driven, easy-to-use sequence alignment editor and sequence analysis program designed and written by a graduate student. This is likely to be the final release of BioEdit. North Carolina State University, Department of Microbiology. ![]()
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